| FL: Ft. Myers-Naples (Florida Direct TV) | | 4 | WEVU | WEVU (IND) | Local |
| 11 | WINK | Programs on CBS include: PGA Championship, NCAA Basketball, CBS Evening News, Ghost Whisperer, Arranged Marriage, The Bridge, Face the Nation, Flashpoint, and 48 Hours. | Local/HD |
| 14 | WTPH | WTPH (AZA) | Local |
| 20 | WBBH | Programs on NBC include: Main article: qubo, Kentucky Derby, The Jay Leno Show, Community, Preakness Stakes, Chuck, The Biggest Loser, Today, and Trauma. | Local/HD |
| 26 | WZVN | Programs on ABC include: Belmont Stakes, America This Morning, ABC World News, Country Music Association Awards, Shark Tank, Wipeout, American Music Awards, Hannah Montana, and Private Practice. | Local/HD |
| 30 | WGCU | Programs on PBS include: Austin City Limits, Caillou, Design e2, Mama Mirabelle's Home Movies, Sesame Street, Super Why!, It's a Big Big World, Ask This Old House, and WordGirl. | Local |
| 36 | WFTX | Programs on FOX include: Human Target, More to Love, So You Think You Can Dance, Lie to Me, American Dad!, Glee, Kitchen Nightmares, Hell's Kitchen, and Family Guy. | Local/HD |
| 46 | WXCW | Programs on CW include: Supernatural, Gossip Girl, The Vampire Diaries, Life Unexpected, America's Next Top Model, One Tree Hill, Melrose Place, Smallville, and 90210. | Local |
| 49 | WRXY | WRXY (IND) | Local |
| FL: Gainesville (Florida Direct TV) |
| FL: Miami-Ft. Lauderdale (Florida Direct TV) | | 2 | WPBT | Programs on PBS include: Austin City Limits, Caillou, Design e2, Mama Mirabelle's Home Movies, Sesame Street, Super Why!, It's a Big Big World, Ask This Old House, and WordGirl. | Local |
| 4 | WFOR | Programs on CBS include: PGA Championship, NCAA Basketball, CBS Evening News, Ghost Whisperer, Arranged Marriage, The Bridge, Face the Nation, Flashpoint, and 48 Hours. | Local/HD |
| 6 | WTVJ | Programs on NBC include: Main article: qubo, Kentucky Derby, The Jay Leno Show, Community, Preakness Stakes, Chuck, The Biggest Loser, Today, and Trauma. | Local/HD |
| 7 | WSVN | Programs on FOX include: Human Target, More to Love, So You Think You Can Dance, Lie to Me, American Dad!, Glee, Kitchen Nightmares, Hell's Kitchen, and Family Guy. | Local/HD |
| 8 | WGEN | WGEN (IND) | Local |
| 10 | WPLG | Programs on ABC include: Belmont Stakes, America This Morning, ABC World News, Country Music Association Awards, Shark Tank, Wipeout, American Music Awards, Hannah Montana, and Private Practice. | Local/HD |
| 17 | WLRN | Programs on PBS include: Austin City Limits, Caillou, Design e2, Mama Mirabelle's Home Movies, Sesame Street, Super Why!, It's a Big Big World, Ask This Old House, and WordGirl. | Local |
| 22 | WSBS | WSBS (IND) | Local |
| 23 | WLTV | WLTV (UNI) | Local |
| 33 | WBFS | WBFS (MNT) | Local |
| 35 | ION | ION (ION) | Local |
| 38 | WPMF | WPMF (AZA) | Local |
| 39 | WSFL | Programs on CW include: Supernatural, Gossip Girl, The Vampire Diaries, Life Unexpected, America's Next Top Model, One Tree Hill, Melrose Place, Smallville, and 90210. | Local |
| 41 | WJAN | WJAN (IND) | Local |
| 51 | WSCV | WSCV (TMO) | Local |
| 69 | WAMI | WAMI (TFT) | Local |
| FL: Orlando-Daytona Beach-Melbourne (Florida Direct TV) | | 2 | WESH | Programs on NBC include: Main article: qubo, Kentucky Derby, The Jay Leno Show, Community, Preakness Stakes, Chuck, The Biggest Loser, Today, and Trauma. | Local/HD |
| 6 | WKMG | Programs on CBS include: PGA Championship, NCAA Basketball, CBS Evening News, Ghost Whisperer, Arranged Marriage, The Bridge, Face the Nation, Flashpoint, and 48 Hours. | Local/HD |
| 9 | WFTV | Programs on ABC include: Belmont Stakes, America This Morning, ABC World News, Country Music Association Awards, Shark Tank, Wipeout, American Music Awards, Hannah Montana, and Private Practice. | Local/HD |
| 15 | WCEU | Programs on PBS include: Austin City Limits, Caillou, Design e2, Mama Mirabelle's Home Movies, Sesame Street, Super Why!, It's a Big Big World, Ask This Old House, and WordGirl. | Local |
| 18 | WKCF | Programs on CW include: Supernatural, Gossip Girl, The Vampire Diaries, Life Unexpected, America's Next Top Model, One Tree Hill, Melrose Place, Smallville, and 90210. | Local |
| 21 | WAU | WAU (AZA) | Local |
| 24 | WMFE | Programs on PBS include: Austin City Limits, Caillou, Design e2, Mama Mirabelle's Home Movies, Sesame Street, Super Why!, It's a Big Big World, Ask This Old House, and WordGirl. | Local |
| 26 | WVEN | WVEN (UNI) | Local |
| 27 | WRDQ | WRDQ (IND) | Local |
| 35 | WOFL | Programs on FOX include: Human Target, More to Love, So You Think You Can Dance, Lie to Me, American Dad!, Glee, Kitchen Nightmares, Hell's Kitchen, and Family Guy. | Local/HD |
| 43 | WOTF | WOTF (TFT) | Local |
| 45 | WTGL | WTGL (IND) | Local |
| 55 | WACX | WACX (IND) | Local |
| 56 | ION | ION (ION) | Local |
| 65 | WRBW | WRBW (MNT) | Local |
| 68 | WBCC | Programs on PBS include: Austin City Limits, Caillou, Design e2, Mama Mirabelle's Home Movies, Sesame Street, Super Why!, It's a Big Big World, Ask This Old House, and WordGirl. | Local |
| FL: Panama City (Florida Direct TV) |
| FL: Tampa-St. Petersburg-Sarasota (Florida Direct TV) | | 3 | WEDU | Programs on PBS include: Austin City Limits, Caillou, Design e2, Mama Mirabelle's Home Movies, Sesame Street, Super Why!, It's a Big Big World, Ask This Old House, and WordGirl. | Local |
| 8 | WFLA | Programs on NBC include: Main article: qubo, Kentucky Derby, The Jay Leno Show, Community, Preakness Stakes, Chuck, The Biggest Loser, Today, and Trauma. | Local/HD |
| 10 | WTSP | Programs on CBS include: PGA Championship, NCAA Basketball, CBS Evening News, Ghost Whisperer, Arranged Marriage, The Bridge, Face the Nation, Flashpoint, and 48 Hours. | Local/HD |
| 13 | WTVT | Programs on FOX include: Human Target, More to Love, So You Think You Can Dance, Lie to Me, American Dad!, Glee, Kitchen Nightmares, Hell's Kitchen, and Family Guy. | Local/HD |
| 16 | WUSF | Programs on PBS include: Austin City Limits, Caillou, Design e2, Mama Mirabelle's Home Movies, Sesame Street, Super Why!, It's a Big Big World, Ask This Old House, and WordGirl. | Local |
| 21 | WAU | WAU (AZA) | Local |
| 22 | WCLF | WCLF (IND) | Local |
| 28 | WFTS | Programs on ABC include: Belmont Stakes, America This Morning, ABC World News, Country Music Association Awards, Shark Tank, Wipeout, American Music Awards, Hannah Montana, and Private Practice. | Local/HD |
| 32 | WMOR | WMOR (IND) | Local |
| 38 | WTTA | WTTA (MNT) | Local |
| 44 | WTOG | Programs on CW include: Supernatural, Gossip Girl, The Vampire Diaries, Life Unexpected, America's Next Top Model, One Tree Hill, Melrose Place, Smallville, and 90210. | Local |
| 50 | WFTT | WFTT (TFT) | Local |
| 62 | WVEA | WVEA (UNI) | Local |
| 66 | WXPX | WXPX (ION) | Local |
| FL: West Palm Beach-Ft. Pierce (Florida Direct TV) | | 5 | WPTV | Programs on NBC include: Main article: qubo, Kentucky Derby, The Jay Leno Show, Community, Preakness Stakes, Chuck, The Biggest Loser, Today, and Trauma. | Local/HD |
| 12 | WPEC | Programs on CBS include: PGA Championship, NCAA Basketball, CBS Evening News, Ghost Whisperer, Arranged Marriage, The Bridge, Face the Nation, Flashpoint, and 48 Hours. | Local/HD |
| 25 | WPBF | Programs on ABC include: Belmont Stakes, America This Morning, ABC World News, Country Music Association Awards, Shark Tank, Wipeout, American Music Awards, Hannah Montana, and Private Practice. | Local/HD |
| 29 | WFLX | Programs on FOX include: Human Target, More to Love, So You Think You Can Dance, Lie to Me, American Dad!, Glee, Kitchen Nightmares, Hell's Kitchen, and Family Guy. | Local/HD |
| 34 | WTVX | Programs on CW include: Supernatural, Gossip Girl, The Vampire Diaries, Life Unexpected, America's Next Top Model, One Tree Hill, Melrose Place, Smallville, and 90210. | Local |
| 41 | WJAN | WJAN (IND) | Local |
| 42 | WXEL | Programs on PBS include: Austin City Limits, Caillou, Design e2, Mama Mirabelle's Home Movies, Sesame Street, Super Why!, It's a Big Big World, Ask This Old House, and WordGirl. | Local |
| 61 | WFGC | WFGC (IND) | Local |
| 67 | ION | ION (ION) | Local |
| 69 | WAMI | WAMI (TFT) | Local |
|
